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Human CKM / CK-MM Protein (His Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Human CKM Protein Product Information
Synonym:CKM, CKMM
Protein Construction:A DNA sequence encoding the human CKM (AAH07462.1) (Met1-Lys381) was expressed with a polyhistide tag at the N-terminus.
Expressed Host:E. coli
Shipping:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Human CKM Protein QC Testing
Purity:> 90 % as determined by SDS-PAGE
Bio-Activity:Kinase activity untested
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:His
Molecule Mass:The recombinant human CKM consists of 396 amino acids and predicts a molecular mass of 44.9 KDa. It migrates as an approximately 45 KDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 100mM HEPES, pH 7.0.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human CKM Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human CKM Protein SDS-PAGE
Human CKM / CK-MM Protein (His Tag) SDS-PAGE
Other CKM Recombinant Protein Products
CKM / CK-MM Background

CKM, also known as CK-MM, is mainly expressed in skeletal muscle. As the primary CK isoenzyme, it also can be detected in heart muscle. CKM is a subunit of creatine kinase (CK). CK is an enzyme expressed by various tissues and cell types. It catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In the cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type).

Human CKM / CK-MM References
  • Goldblatt H. 1969, Laboratory Investigation. 21 (2): 126-8.
  • Hekimsoy Zeliha. et al., 2005, Endocrine Research. 31 (3): 171-5.
  • Döring F. et al., 2011, Scand J Med Sci Sports. 21(6):841-5.
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    Size / Price
    Catalog: 15076-H07E-50
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