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Human DCUN1D1 / SCCRO Protein (His Tag)

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Human DCUN1D1 Protein Product Information
Synonym:DCUN1D1, DCUN1L1, RP42, SCCRO
Protein Construction:A DNA sequence encoding the human DCUN1D1 (NP_065691.2) (Met1-Val259) was expressed with a polyhistidine tag at the N-terminus.
Expressed Host:E. coli
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human DCUN1D1 Protein QC Testing
Purity:> 90 % as determined by SDS-PAGE
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:His
Molecule Mass:The recombinant human DCUN1D1 consists of 274 amino acids and predicts a molecular mass of 32 KDa. It migrates as an approximately 28-32 KDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, 10% glycerol, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human DCUN1D1 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human DCUN1D1 Protein SDS-PAGE
Human DCUN1D1 / SCCRO Protein (His Tag) SDS-PAGE
Other DCUN1D1 Recombinant Protein Products
DCUN1D1 / SCCRO Background

DCUN1D1, also known as SCCRO, is part of an E3 ubiquitin ligase complex for neddylation. DCUN1D1 functions to recruit charged E2 and is involved in the release of inhibitory effects of CAND1 on cullin-RING ligase E3 complex assembly and activity. DCUN1D1 binds to the components of the neddylation pathway (Cullin-ROC1, Ubc12, and CAND1) and augments but is not required for cullin neddylation in reactions using purified recombinant proteins. DCUN1D1 also recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation in purified protein assays. DCUN1D1 also acts as am ncogene facilitating malignant transformation and carcinogenic progression.

Human DCUN1D1 / SCCRO References
  • Heir P. et al., 2013, Mol Cell Biol. 33 (8): 1621-31.
  • Kurz T. et al., 2005, Nature. 435 (7046): 1257-61.
  • Kim AY. et al., 2008, J Biol Chem. 283 (48): 33211-20.
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    Catalog: 15059-H07E-20
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