|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse UBE2I ORF mammalian expression plasmid, C-GFPSpark tag||MG52988-ACG|
|Mouse UBE2I ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52988-ACR|
|Mouse UBE2I ORF mammalian expression plasmid, C-Flag tag||MG52988-CF|
|Mouse UBE2I ORF mammalian expression plasmid, C-His tag||MG52988-CH|
|Mouse UBE2I ORF mammalian expression plasmid, C-Myc tag||MG52988-CM|
|Mouse UBE2I ORF mammalian expression plasmid, C-HA tag||MG52988-CY|
|Mouse UBE2I Gene cDNA clone plasmid||MG52988-G|
|Mouse UBE2I ORF mammalian expression plasmid, N-Flag tag||MG52988-NF|
|Mouse UBE2I ORF mammalian expression plasmid, N-His tag||MG52988-NH|
|Mouse UBE2I ORF mammalian expression plasmid, N-Myc tag||MG52988-NM|
|Mouse UBE2I ORF mammalian expression plasmid, N-HA tag||MG52988-NY|
|Mouse UBE2I natural ORF mammalian expression plasmid||MG52988-UT|
|Learn more about expression Vectors|
UBE2I is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.