|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse LYPLA2 ORF mammalian expression plasmid, C-GFPSpark tag||MG52835-ACG|
|Mouse LYPLA2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52835-ACR|
|Mouse LYPLA2 ORF mammalian expression plasmid, C-Flag tag||MG52835-CF|
|Mouse LYPLA2 ORF mammalian expression plasmid, C-His tag||MG52835-CH|
|Mouse LYPLA2 ORF mammalian expression plasmid, C-Myc tag||MG52835-CM|
|Mouse LYPLA2 ORF mammalian expression plasmid, C-HA tag||MG52835-CY|
|Mouse LYPLA2 Gene cDNA clone plasmid||MG52835-G|
|Mouse LYPLA2 ORF mammalian expression plasmid, N-Flag tag||MG52835-NF|
|Mouse LYPLA2 ORF mammalian expression plasmid, N-His tag||MG52835-NH|
|Mouse LYPLA2 ORF mammalian expression plasmid, N-Myc tag||MG52835-NM|
|Mouse LYPLA2 ORF mammalian expression plasmid, N-HA tag||MG52835-NY|
|Mouse LYPLA2 natural ORF mammalian expression plasmid||MG52835-UT|
|Learn more about expression Vectors|
Lysophospholipase II (LYPLA2, LPL-II, or LysoPLA II), also known as Acyl-protein thioesterase 2 (APT-2), belongs to the AB hydrolase 2 family. This enzyme has lysophospholipase activity, and may hydrolyze fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acyl-protein thioesterase 1 (APT-1) and Acyl-protein thioesterase 2 (APT-2) are cytosolic lysophospholipid hydrolyzing enzymes. The serum activity of APT-1 may play an important role in determination of the concentration of des-acyl ghrelin in circulation, especially under septic inflammation. APT-2/LYPLA2 is expressed both in CHO-K1 and HeLa cells and its overexpression increased the deacylation rate of single acylated GAP-43 and affected the steady-state localization of diacylated GAP-43 and H-Ras. Thus, the results demonstrate that APT-2/LYPLA2 is the protein thioesterase involved in the acylation/deacylation cycle operating in GAP-43 subcellular distribution.