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Human ARF5 / ADP-ribosylation factor 5 Protein (His Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
ARF5Protein Product Information
Synonym:ARF5
Protein Construction:A DNA sequence encoding the human ARF5 (AAP36805.1)(Met1-Arg180) was expressed with a polyhistidine tag at the N-terminus.
Species:Human
Expressed Host:E. coli
Form & Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
ARF5Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:His
Molecule Mass:The recombinant human ARF5 consists of 198 amino acids and predicts a molecular mass of 22.8 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, 10% glycerol, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
ARF5Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
14934-H07E
Background

ARF5, also known as ADP-ribosylation factor 5, belongs to the small GTPase superfamily, Arf family. Members of this family stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. ARF5 functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. ARF5 Is involved in protein trafficking. ARF5 may also modulate vesicle budding and uncoating within the Golgi apparatus.

References
  • Kanoh H. et al., 1997, J Biol Chem. 272 (9): 5421-9.
  • Tsuchiya M. et al., 1991, J Biol Chem. 266 (5): 2772-7.
  • McGuire RE. et al., 1997, Genomics. 41 (3): 481-4.
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    Catalog:14934-H07E-20
    List Price: $108.00  (Save $0.00)
    Price:$108.00      [How to order]
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    • Human ARF5 / ADP-ribosylation factor 5 Protein (His Tag) SDS-PAGE
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