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Human WARS / TrpRS Protein (His Tag)

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Human WARS Protein Product Information
Synonym:WARS, IFI53, WRS
Protein Construction:A DNA sequence encoding the human WARS (P23381-1) (Pro2-Gln471) was fused with a polyhistide tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human WARS Protein QC Testing
Purity:> 90 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:His
Molecule Mass:The recombinant human WARS consists of 487 amino acids and has a calculated molecular mass of 55.2 kDa. The recombinant protein migrates as an approximately 55 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human WARS Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other WARS Recombinant Protein Products
WARS / TrpRS Background

WARS, also known as TrpRS, is an aminoacyl-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. There are two forms of tryptophanyl-tRNA synthetase: a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. They catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. WARS catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon.

Human WARS / TrpRS References
  • Buwitt U. et al., 1992, EMBO J. 11 (2): 489-96.
  • Fleckner J. et al., 1992, Proc Natl Acad Sci. 88 (24): 11520-4.
  • Ewalt KL. et al., 2002, Biochemistry. 41 (45): 13344-9.
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    Catalog: 14827-H07B-20
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