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Human ALOX15B / 15 Lipoxygenase 2 Protein (His & GST Tag)

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Human ALOX15B Protein Product Information
Protein Construction:A DNA sequence encoding the human ALOX15B (AAH35217.1)( Met1-Ile676) was expressed the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human ALOX15B Protein QC Testing
Purity:> 90 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The secreted recombinant human ALOX15B consists of 913 amino acids and predicts a molecular mass of 103.7 KDa. The apparent molecular mass of the protein is approximately 93 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation:Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human ALOX15B Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other ALOX15B Recombinant Protein Products
ALOX15B / 15 Lipoxygenase 2 Background

ALOX15B is a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. ALOX15B converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. ALOX15B gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.

Human ALOX15B / 15 Lipoxygenase 2 References
  • Kilty I. et al., 2000, Eur J Biochem. 266 (1): 83-93.
  • Sigal E. et al., 1990, J Biol Chem. 265 (9): 5113-20.
  • Brash AR. et al., 1997, Proc Natl Acad Sci. 94 (12): 6148-52.
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    Size / Price
    Catalog: 14564-H20B-100
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