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Human Discoidin / DCBLD1 Protein (His Tag)

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DCBLD1Protein Product Information
Synonym:DCBLD1
Protein Construction:A DNA sequence encoding the human DCBLD1 (Q8N8Z6-2) (Met1-Val459) was expressed with a polyhistidine tag at the C-terminus.
Species:Human
Expressed Host:Human Cells
Form & Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
DCBLD1Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Glu 35
Molecule Mass:The recombinant human DCBLD1 consists of 436 amino acids and predicts a molecular mass of 48.4 KDa. It migrates as an approximately 65 KDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
DCBLD1Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
14558-H08H
Background

Discoidin, also known as DCBLD1, contains 1 CUB domain, 1 F5/8 type C domain and 1 LCCL domain. It is a 715 amino acid single-pass membrane protein. Discoidin has a discoidin I-like domain which shares a common C-terminal region of about 110 amino acids with the FA58C domain, but whose N-terminal 40 amino acids are much less conserved. Discoidin gene contains 31 distinct gt-ag introns. Transcription produces 18 different mRNAs, 16 alternatively spliced variants and 2 unspliced forms. Discoidin is expected to localize in various compartments.

References
  • The MGC Project Team. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14:2121-27.
  • Horton R, et al. (2003) The DNA sequence and analysis of human chromosome 6. Nature 425:805-11.
  • Suzuki Y, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36:40-5.
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    Catalog:14558-H08H-20
    List Price: $158.00  (Save $0.00)
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    • Human Discoidin / DCBLD1 Protein (His Tag) SDS-PAGE
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