|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse C1D ORF mammalian expression plasmid, C-GFPSpark tag||MG52866-ACG|
|Mouse C1D ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52866-ACR|
|Mouse C1D ORF mammalian expression plasmid, N-GFPSpark tag||MG52866-ANG|
|Mouse C1D ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52866-ANR|
|Mouse C1D ORF mammalian expression plasmid, C-Flag tag||MG52866-CF|
|Mouse C1D ORF mammalian expression plasmid, C-His tag||MG52866-CH|
|Mouse C1D ORF mammalian expression plasmid, C-Myc tag||MG52866-CM|
|Mouse C1D ORF mammalian expression plasmid, C-HA tag||MG52866-CY|
|Mouse C1D Gene cDNA clone plasmid||MG52866-G|
|Mouse C1D ORF mammalian expression plasmid, N-Flag tag||MG52866-NF|
|Mouse C1D ORF mammalian expression plasmid, N-His tag||MG52866-NH|
|Mouse C1D ORF mammalian expression plasmid, N-Myc tag||MG52866-NM|
|Mouse C1D ORF mammalian expression plasmid, N-HA tag||MG52866-NY|
|Mouse C1D natural ORF mammalian expression plasmid||MG52866-UT|
|Learn more about expression Vectors|
C1D nuclear receptor corepressor belongs to the C1D family. It is a DNA binding and apoptosis-inducing protein.C1D nuclear receptor corepressorinteracts with TSNAX and DNA-PKcs. It acts as a corepressor for the thyroid hormone receptor. It is thought that C1D nuclear receptor corepressor regulates TRAX/Translin complex formation. It is expressed in kidney, heart, brain, spleen, lung, testis, liver and small intestine. It plays a role in the recruitment of the RNA exosome complex to pre-rRNA to mediate the 3'-5' end processing of the 5.8S rRNA; this function may include MPHOSPH6. It potentiates transcriptional repression by NR1D1 and THRB. C1D nuclear receptor corepressorcan activate PRKDC not only in the presence of linear DNA but also in the presence of supercoiled DNA. It also can induce apoptosis in a p53/TP53 dependent manner.