|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse ENO3 ORF mammalian expression plasmid, C-GFPSpark tag||MG52862-ACG|
|Mouse ENO3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52862-ACR|
|Mouse ENO3 ORF mammalian expression plasmid, C-Flag tag||MG52862-CF|
|Mouse ENO3 ORF mammalian expression plasmid, C-His tag||MG52862-CH|
|Mouse ENO3 ORF mammalian expression plasmid, C-Myc tag||MG52862-CM|
|Mouse ENO3 ORF mammalian expression plasmid, C-HA tag||MG52862-CY|
|Mouse ENO3 Gene cDNA clone plasmid||MG52862-G|
|Mouse ENO3 ORF mammalian expression plasmid, N-Flag tag||MG52862-NF|
|Mouse ENO3 ORF mammalian expression plasmid, N-His tag||MG52862-NH|
|Mouse ENO3 ORF mammalian expression plasmid, N-Myc tag||MG52862-NM|
|Mouse ENO3 ORF mammalian expression plasmid, N-HA tag||MG52862-NY|
|Mouse ENO3 natural ORF mammalian expression plasmid||MG52862-UT|
|Learn more about expression Vectors|
ENO3 is one of the three enolase isoenzymes found in mammals. As a homodimer, ENO3 is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for ENO3 gene that differ only in their 5' UTR. ENO3 may play a role in muscle development and regeneration. It appears to have a function in striated muscle development and regeneration.