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Cynomolgus monkey UBA1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-tagged

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UBA1cDNA Clone Product Information
Gene_bank_ref_id:unsubmitted
cDNA Size:3177
cDNA Description:ORF Clone of Macaca fascicularis ubiquitin-like modifier activating enzyme 1DNA.
Gene Synonym:UBA1
Species:Cynomolgus
Vector:pCMV3-N-His
Restriction Site:
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pCMV3-N-His Vector Information
 
Vector Name pCMV3-N-His
Vector Size 6104bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag His
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV3-N-His Physical Map

Schematic of pCMV3-N-His Multiple Cloning Sites

His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Cynomolgus monkey UBA1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-tagged on other vectors
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Cynomolgus monkey UBA1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagCG90821-ANR$425
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Cynomolgus monkey UBA1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedCG90821-CM$395
Cynomolgus monkey UBA1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedCG90821-CY$395
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Cynomolgus monkey UBA1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedCG90821-NF$395
Cynomolgus monkey UBA1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedCG90821-NH$395
Cynomolgus monkey UBA1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedCG90821-NM$395
Cynomolgus monkey UBA1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedCG90821-NY$395
Cynomolgus monkey UBA1 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedCG90821-UT$395
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Background

UBE1, also known as UBA1, belongs to the ubiquitin-activating E1 family. UBE1 gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It also catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Defects in UBA1 can cause spinal muscular atrophy X-linked type 2 (SMAX2), also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.

References
  • Jin J, et al. (2007) Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature. 447(7148):1135-8.
  • Xia T, et al. (2007) Chaperone-dependent E3 ligase CHIP ubiquitinates and mediates proteasomal degradation of soluble guanylyl cyclase. Am J Physiol Heart Circ Physiol. 293(5):H3080-7.
  • Pridgeon JW, et al. (2009) Proteomic analysis reveals Hrs UIM-mediated ubiquitin signaling in multiple cellular processes. FEBS J. 276(1):118-31.
  • Size / Price
    Catalog:CG90821-NH
    List Price: $395.00  (Save $0.00)
    Price:$395.00      [How to order]
    Availability2-3 weeks
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