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Human ADCYAP1R1 HEK293 Cell Lysate (WB positive control)

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Human ADCYAP1R1 Transfected / Overexpression Cell Lysate Product Information
Expressed Host:Human Cells
Product Description:Human Cell lysate that Human ADCYAP1R1 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
Sequence information:A DNA sequence encoding the human ADCYAP1R1 (P41586) extracellular domain (Met 1-Ala 155) was fused with the Fc region of human IgG1 at the C-terminus.
Predicted N Terminal:Asp 24
Molecule Mass:The recombinant human ADCYAP1R1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 373 amino acids and has a predicted molecular mass of 42 kDa. The reduced monomer migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions due to glycosylation.
Human ADCYAP1R1 Transfected / Overexpression Cell Lysate Usage Guide
Preparation Method:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
Stability:Samples are stable for up to twelve months from date of receipt.
Recommend Usage:1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
Storage Buffer:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Storage Instruction:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
Application notes:Western blot (WB): Use at an assay dependent dilution.
Other Applications: Not tested.
Optimal dilutions/concentrations should be determined by the end user.
Human ADCYAP1R1 References
  • Solano RM. et al., 1996, Endocrinology. 137 (7): 2815-22.
  • Miampamba M. et al., 2002, Regul Pept. 105 (3): 145-54.
  • Brabet P. et al., 1997, Genomics. 38 (1): 100-2.
  • Size / Price
    Catalog: 13223-H02HL-300
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