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狗 MUT 基因全长ORF克隆

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Canine MUT cDNA Clone Product Information
NCBI RefSeq:XM_532164.4
RefSeq ORF Size:450bp
cDNA Description:Full length Clone DNA of Canis lupus familiaris methylmalonyl CoA mutase.
Gene Synonym:MUT
Species:Canine
Vector:pUC19 Vector
Plasmid:cpUC19-dMUT
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 597C/T and 2022C/T not causing the amino acid variation; 272T/C(F91S), 395A/G(Q132R), 1124T/C(M375T), 1967A/G(E656G).
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
pUC19 vector Vector Information:

pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.

pUC19 vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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Catalog: DG70241-U
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