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Human PTPRA / PTPalpha Protein (aa 174-793, His & GST Tag)

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Human PTPRA Protein Product Information
Synonym:RP4-534B8.1, HEPTP, HLPR, HPTPA, HPTPalpha, LRP, PTPA, PTPRL2, R-PTP-alpha, RPTPA
Protein Construction:A DNA sequence encoding the human PTPRA isoform 2 (P18433-2) cytoplasmic domain (Ala 174-Lys 793) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human PTPRA Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:1. Measured by its ability to bind biotinylated recombinant mouse SRC in a functional ELISA.
2. Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >40000 pmol/min/μg.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human PTPRA (aa 174-793)/GST chimera consists of 857 amino acids and has a calculated molecular mass of 99 kDa. It migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 20% gly, 3mM DTT
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human PTPRA Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human PTPRA Protein SDS-PAGE
Human PTPRA / PTPalpha Protein (aa 174-793, His & GST Tag) SDS-PAGE
Other PTPRA Recombinant Protein Products
Human PTPRA References
  • Kaplan R, et al. (1990) Cloning of three human tyrosine phosphatases reveals a multigene family of receptor-linked protein-tyrosine-phosphatases expressed in brain. Proc Natl Acad Sci U S A. 87 (18): 7000-4.
  • Hertog JD, et al. (1996) Tight association of GRB2 with receptor protein-tyrosine phosphatase alpha is mediated by the SH2 and C-terminal SH3 domains. EMBO J. 15 (12): 3016-27.
  • Ye H, et al. (2011) Receptor-like protein-tyrosine phosphatase a enhances cell surface expression of neural adhesion molecule NB-3. J Biol Chem. 286 (29): 26071-80.
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    Catalog: 13175-H20B-20
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