After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

Human PGDH / PHGDH Protein (His Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Human PHGDH Protein Product Information
Synonym:RP4-683H9.1, 3-PGDH, 3PGDH, PDG, PGAD, PGD, PGDH, SERA
Protein Construction:A DNA sequence encoding the mature form of human PHGDH (O43175) (Met 1-Phe 533) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
Expressed Host:E. coli
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human PHGDH Protein QC Testing
Purity:> 90 % as determined by SDS-PAGE
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human PHGDH comprises 543 amino acids and has a calculated molecular mass of 58KDa. It migrates as an approximately 55 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, 100mM Arg, 0.1% Tween20, 20% glycerol, pH 8.0
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human PHGDH Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human PHGDH Protein SDS-PAGE
Human PGDH / PHGDH Protein (His Tag) SDS-PAGE
Other PHGDH Recombinant Protein Products
PGDH/PHGDH Background

PHGDH is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family. This new family consists of D-isomer-stereospecific enzymes. The conserved residues in this family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.

Human PGDH/PHGDH References
  • Pind S, et al. (2002) V490M, a common mutation in 3-phosphoglycerate dehydrogenase deficiency, causes enzyme deficiency by decreasing the yield of mature enzyme. J Biol Chem. 277 (9): 7136-43.
  • Du H, et al. (2010) 3-Phosphoglycerate dehydrogenase expression is regulated by HOXA10 in murine endometrium and human endometrial cells. Reproduction. 139 (1): 237-45.
  • Possemato R, et al. (2011) Functional genomics reveal that the serine synthesis pathway is essential in breast cancer. Nature. 476 (7360): 346-50.
  • Product nameProduct name
    Size / Price
    Catalog: 13167-H08E-20
    List Price:   (Save )
    Price:      [How to order]
    AvailabilityIn StockShipping instructions