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RNase A / Ribonuclease A / RNASE1 Antibody, Mouse MAb

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Human RNASE1 Antibody Product Information
Immunogen:Recombinant Human RNase A / Ribonuclease A / RNASE1 protein (Catalog#13468-H08H)
Clone ID:02
Ig Type:Mouse IgG1
Formulation:0.2 μm filtered solution in PBS
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RNase A / Ribonuclease A / RNASE1 (rh RNase A / Ribonuclease A / RNASE1; Catalog#13468-H08H; P07998; Met1-Thr156). The IgG fraction of the cell culture supernatant was purified by Protein A affinity.
Human RNASE1 Antibody Usage Guide
Specificity:Human RNase A / Ribonuclease A / RNASE1
No cross-reactivity in ELISA with
Human cell lysate (293 cell line)

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human RNase A / Ribonuclease A / RNASE1. The detection limit for Human RNase A / Ribonuclease A / RNASE1 is approximately 0.31 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other RNASE1 Antibody Products
RNase A / Ribonuclease A / RNASE1 Background

RNase A, also known as ribonuclease A and RNASE1, belongs to ribonuclease A superfamily. It is a pancreatic-type of secretory ribonuclease. RNase A is a basic protein and its many positive charges are consistent with its binding to RNA (a poly-anion). More generally, RNase A is unusually polar or, rather, unusually lacking in hydrophobic groups, especially aliphatic ones. As a endonuclease, RNase A cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. RNase A is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

Human RNase A / Ribonuclease A / RNASE1 References
  • Tubert P, et al. (2011) Interactions crucial for three-dimensional domain swapping in the HP-RNase variant PM8. Biophys J. 101(2):459-67.
  • Vinayagam A, et al. (2011) A directed protein interaction network for investigating intracellular signal transduction. Sci Signal. 4(189):rs8.
  • Fischer S, et al. (2011) Expression and localisation of vascular ribonucleases in endothelial cells. Thromb Haemost. 105(2):345-55.
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