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LC3B / MAP1LC3B Antibody, Mouse MAb

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Human MAP1LC3B Antibody Product Information
Immunogen:Recombinant Human LC3B / MAP1LC3B protein (Catalog#14555-H07E)
Clone ID:01
Ig Type:Mouse IgG2a
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LC3B / MAP1LC3B (rh LC3B / MAP1LC3B; Catalog#14555-H07E; Q9GZQ8; Met1-Val125). The IgG fraction of the cell culture supernatant was purified by Protein A affinity.
Human MAP1LC3B Antibody Usage Guide
Specificity:Human LC3B / MAP1LC3B
No cross-reactivity in ELISA with
E.coli cell lysate
Application:ELISA

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human LC3B / MAP1LC3B. The detection limit for Human LC3B / MAP1LC3B is approximately 0.16 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other MAP1LC3B Antibody Products
LC3B / MAP1LC3B Background

LC3B, also known as MAP1LC3B, is a member of the MAP1 LC3 family. It is moat abundantly expressed in heart, brain, skeletal muscle and testis. LC3B is a subunit of neuronal microtubule and functions in formation of autophagosomal vacuoles (autophagosomes). It associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. LC3B also plays a role in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Human LC3B / MAP1LC3B References
  • Behrends C. et al., 2010, Nature. 466 (7302): 68-76.
  • Tanida I. et al., 2005, Int J Biochem Cell Biol. 36 (12): 2503-18.
  • Kabeya Y. et al., 2000, EMBO J. 19 (21): 5720-8.
  • Cherra SJ. et al., 2010, J Cell Biol. 190 (4): 533-9.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"