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PS6K / RPS6KB1 Antibody, Mouse MAb

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Human RPS6KB1 Antibody Product Information
Immunogen:Recombinant Human PS6K / RPS6KB1 protein (Catalog#10099-H09B)
Clone ID:05
Ig Type:Mouse IgG2a
Formulation:0.2 μm filtered solution in PBS
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PS6K / RPS6KB1 (rh PS6K / RPS6KB1; Catalog#10099-H09B; P23443; Met1-Leu525). The IgG fraction of the cell culture supernatant was purified by Protein A affinity.
Human RPS6KB1 Antibody Usage Guide
Specificity:Human PS6K / RPS6KB1
No cross-reactivity in ELISA with
Insect cell lysate

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human PS6K / RPS6KB1. The detection limit for Human PS6K / RPS6KB1 is approximately 0.078 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other RPS6KB1 Antibody Products
PS6K / RPS6KB1 Background

PS6K, also known as RPS6KB1, is a serine/threonine-protein kinase. It belongs to the RSK (ribosomal s6 kinase) family. Members of this family function in signal transduction. PS6K is an isoform of p70 ribosomal S6 kinase (S6K). S6K can be activated by mitogenic stimuli such as growth factors, insulin and cytokines. It phosphorylates the ribosomal protein S6. PS6K also phosphorylates other proteins such as elF4B, eEF2K and SKAR. It is a crucial effector of mTOR(rapamycin) signaling. PS6K is dissociated from the EIF3 complex and activated upon mitogenic stimulation, phosphorylation by the mammalian target of mTOR complex 1 (mTORC1). Its active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. PS6K also functions in cell proliferation, cell growth and cell cycle progression.

Human PS6K / RPS6KB1 References
  • Panasyuk, et al. (2006) Nuclear export of PS6K II is regulated by protein kinase CK2 phosphorylation at Ser-17. J Biol Chem. 281(42):31188-201.
  • Carnevalli L, et al. (2010) PS6K Plays a Critical Role in Early Adipocyte Differentiation. Dev Cell. 18 (5):763-74.
  • Grove JR, et al. (1991) Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini. Mol Cell Biol. 11(11):5541-50.
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