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Human SLITRK4 / Slitrk4 HEK293 Cell Lysate (WB positive control)

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Human SLITRK4 Transfected / Overexpression Cell Lysate Product Information
Expressed Host:Human Cells
Product Description:Human Cell lysate that Human SLITRK4 / Slitrk4 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
Sequence information:A DNA sequence encoding the human SLITRK4 (Q8IW52) (Met 1-Pro 616) was fused with a polyhistidine tag at the C-terminus.
Predicted N Terminal:Asp 19
Molecule Mass:The secreted recombinant human SLITRK4 comprises 609 amino acids and has a predicted molecular mass of 68.6 kDa. The apparent molecular mass of rhSLITRK4 is approximately 85 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Human SLITRK4 Transfected / Overexpression Cell Lysate Usage Guide
Preparation Method:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
Stability:Samples are stable for up to twelve months from date of receipt.
Recommend Usage:1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
Storage Buffer:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Storage Instruction:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
Application notes:Western blot (WB): Use at an assay dependent dilution.
Other Applications: Not tested.
Optimal dilutions/concentrations should be determined by the end user.
SLITRK4 Background

SLIT and NTRK-like family proteins 4 is a member of the SLIT and NTRK-like family.This protein is characterized as an integral membrane protein that has two leucine-rich repeat (LRR) domains and a carboxy-terminal domain that is partially similar to SLIT protein andTRK Neurotrophin There are six members in this family: SLITRK1, SLITRK2, SLITRK3, SLITRK4, SLITRK5, and SLITRK6. Their expression was detected mainly in the brain, but the expression profile of each SLITRK was unique. Each of these members is located in nervous system except for SLITRK6. SLITRK expression was also investigated in various types of brain tumor tissue. 

Human SLITRK4 References
  • Beaubien F, et al. (2009) Differential expression of slitrk family members in the mouse nervous system. Developmental Dynamics. 238 (12) : 3285-96.
  • Aruga J, et al. (2003) Human SLITRK family genes: genomic organization and expression profiling in normal brain and brain tumor tissue. Gene. 315 (2): 87-94.
  • Milde T, et al. (2007) A novel family of slitrk genes is expressed on hematopoietic stem cells and leukemias. Leukemia. 21: 824-7.
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    Catalog: 13147-H08HL-300
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