|Datasheet||Specific References||Reviews||Related Products||Protocols|
|0.5 mg/mL of rabbit anti-mTHSD1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1 μg/mL in PBS before coating.|
|0.25 mg/mL rabbit anti- mTHSD1 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use.|
|Each vial contains 95 ng of recombinant mTHSD1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.|
|The minimum detectable dose of Mouse THSD1 / TMTSP was determined to be approximately 62.500 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|The Mouse THSD1 / TMTSP ELISA Pair Set is for the quantitative determination of Mouse THSD1 / TMTSP. |
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for THSD1 / TMTSP coated on a 96-well plate. Standards and samples are added to the wells, and any THSD1 / TMTSP present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-THSD1 / TMTSP polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of THSD1 / TMTSP present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
|Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.|
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Thrombospondin type-1 domain-containing protein 1, also known as transmembrane molecule with thrombospondin module, THSD1 and TMTSP, is a single-pass type I membrane protein which contains one TSP type-1 domain. THSD1 is a multi-domain, multi-functional glycoprotein synthesized by many cells. Matricellular THSD1 modulates cell adhesion and proliferation. It is involved in angiogenesis, inflammation, wound healing and cancer. In vitro, nanomolar concentrations of Thrombospondin-1 are required to alter endothelial and vascular smooth muscle cell adhesion, proliferation, motility, and survival. As a major platelet protein, for a long time it was postulated to control hemostasis via platelet aggregate stabilization. THSD1 is a potent angiogenesis inhibitor, and down-regulation of THSD1 has been suggested to alter tumor growth by modulating angiogenesis in a variety of tumor types.