|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Recombinant Aequorea victoria GFP protein (Catalog#13105-S07E)|
|0.2 μm filtered solution in PBS with 5% trehalose|
|This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Aequorea victoria GFP (Catalog#13105-S07E; AAB65663; Ser 2-Lys 238). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.|
|Aequorea victoria GFP|
No cross-reactivity in ELISA with
E.coli cell lysate
|ELISA, IP, ICC/IF, IF|
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Aequorea victoria GFP. The detection limit for Aequorea victoria GFP is approximately 0.16 ng/well.
ICC/IF: 10-25 μg/mL
IP: 1-4 μg/mg of lysate
|This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.|
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.