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Human IGFBP7 / IBP-7 Protein (Fc Tag)

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Human IGFBP7 Protein Product Information
Protein Construction:A DNA sequence encoding the human IGFBP7 (Q16270) (Met 1-Leu 282) was fused with the Fc region of human IgG1 at the C-terminus.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human IGFBP7 Protein QC Testing
Purity:> 85 % as determined by SDS-PAGE
Bio-Activity:Measured by its binding ability in a functional ELISA .
1. Immobilized IGFBP7-Fc at 20 μg/ml (100 μl/well) can bind biotinylated human IGF2-nusa. The EC50 of biotinylated human IGF2-nusa is 0.06 μg/ml .
2. Immobilized human CCL21 at 2 μg/ml (100 μl/well) can bind human IGFBP7-Fc with a linear ranger of 0.16-4 μg/ml.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Ser 27
Molecule Mass:The secreted recombinant human IGFBP7/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 497 amino acids and has a predicted molecular mass of 53.5 kDa. The apparent molecular mass of rh IGFBP7/Fc monomer is approximately 65-70 kDa in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human IGFBP7 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other IGFBP7 Recombinant Protein Products
IGFBP7 Background

Insulin like growth factor binding protein 7 (IGFBP7) is a member of the IGFBP family. It has been identified in colorectal adenocarcinoma (CRC) cell lines. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are clearly distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF, or act in an IGF-independent manner in the prostate. IGFBP7 could inhibit cell growth, decrease soft agar colony formation activity and induce apoptosis in RKO and SW620 cells. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence and IGF affinity.

Human IGFBP7 References
  • Oh Y, et al. (1996) Synthesis and characterization of insulin-like growth factor-binding protein (IGFBP)-7. Recombinant human mac25 protein specifically binds IGF-I and -II. J Biol Chem. 271(48): 30322-5.
  • Wilson EM, et al. (1997) Generation and characterization of an IGFBP-7 antibody: identification of 31kD IGFBP-7 in human biological fluids and Hs578T human breast cancer conditioned media. J Clin Endocrinol Metab. 82(4): 1301-3.
  • Lin J, et al. (2007) Methylation patterns of IGFBP7 in colon cancer cell lines are associated with levels of gene expression. J Pathol. 212(1): 83-90.
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    Catalog: 13100-H02H-20
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