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Human TLE3 Protein (aa 484-772, GST Tag)

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Human TLE3 Protein Product Information
Synonym:ESG, ESG3, FLJ39460, GRG3, HsT18976, KIAA1547
Protein Construction:A DNA sequence encoding the N-terminal fragment of human TLE3 (Q04726-1) (Ser 484-Tyr 772) was fused with the GST tag at the N-terminus.
Expressed Host:E. coli
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human TLE3 Protein QC Testing
Purity:> 78 % as determined by SDS-PAGE
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human TLE3/GST chimera consists of 520 amino acids and has a predicted molecular mass of 58.5 kDa as estimated in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 20mM Tris, 0.15M NaCl, 5mM GSH, pH 7.5
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human TLE3 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human TLE3 Protein SDS-PAGE
Human TLE3 Protein (aa 484-772, GST Tag) SDS-PAGE
Other TLE3 Recombinant Protein Products
Human TLE3 References
  • Villanueva CJ, et al. (2011) TLE3 is a dual-function transcriptional coregulator of adipogenesis. Cell Metab. 13(4): 413-27.
  • Carvalho LR, et al. (2010) Corepressors TLE1 and TLE3 interact with HESX1 and PROP1. Mol Endocrinol. 24(4): 754-65.
  • Nakaya HI, et al. (2007) Splice variants of TLE family genes and up-regulation of a TLE3 isoform in prostate tumors. Biochem Biophys Res Commun. 364(4): 918-23.
  • Brinkmeier ML, et al. (2003) TCF and Groucho-related genes influence pituitary growth and development. Mol Endocrinol. 17: 2152-2161.
  • Dasen JS, et al. (2001) Temporal regulation of a paired-like homeodomain repressor/TLE corepressor complex and a related activator is required for pituitary organogenesis. Genes Dev. 15: 3193-3207.
  • Douglas KR, et al. (2001) Identification of members of the Wnt signaling pathway in the embryonic pituitary gland. Mamm Genome. 12: 843-851.
  • Chen G, et al. (2000) Groucho/TLE family proteins and transcriptional repression. Gene. 249: 1-16.
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    Catalog: 13099-H09E-100
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    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.