|Datasheet||Specific References||Reviews||Related Products||Protocols|
|A DNA sequence encoding the E.Coli ssPA (P0ACA3) (Met 1-Ser 212) was expressed, with a polyhistidine tag at the N-terminus.|
|In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|> 97 % as determined by SDS-PAGE|
|Please contact us for more information.|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|The recombinant E.Coli ssPA consisting of 228 amino acids and has a calculated molecular mass of 26.4 kDa. It migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.|
|Lyophilized from sterile PBS, pH 7.4|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
Stringent starvation protein A (ssPA), a member of the GST superfamily, is an RNA polymerase-associated transcriptional activator for the lytic development of phage P1 and is essential for stationary phase-induced acid tolerance of E. coli. It indicate that the flexible regions are not critical for SspA function, whereas the surface pocket is important for both transcriptional activation of the phage P1 late promoter and acid resistance of E. coli. SspA is known to be implicated in survival during nutrient starvation and prolonged stationary phase. Recently, SspA was shown to play an important role in the stationary phase-induced stress response including acid tolerance by down-regulating the level of the global regulator H-NS, which negatively regulates multiple stress defense systems.