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Rat PGK1 Gene cDNA Clone (full-length ORF Clone)

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PGK1cDNA Clone Product Information
Gene_bank_ref_id:NM_053291.3
cDNA Size:1254
cDNA Description:ORF Clone of Rattus norvegicus phosphoglycerate kinase 1 DNA.
Gene Synonym:Pgk
Species:Rat
Vector:pUC19 Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pUC19 vector Vector Information:

pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.

pUC19 vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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References
  • Rush J. et al., 2005, Nat Biotechnol. 23: 94-101.
  • Olsen JV. et al., 2006, Cell. 127: 635-648.
  • Zieker D. et al., 2008, Cell Physiol Biochem. 21 (5-6): 429-36.
  • Jung Y. et al., 2009, Mol Cancer Res. 7 (10): 1595-604.
  • Choudhary C. et al., 2009, Science. 325: 834-40.
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    Catalog:RG80663-U
    List Price: $95.00  (Save $0.00)
    Price:$95.00      [How to order]
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    • Rat PGK1 Gene cDNA Clone (full-length ORF Clone)
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