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Hepatitis C virus (HCV-1a) NS5A ORF mammalian expression plasmid, N-His tag

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HCV HCV-NS5A cDNA Clone Product Information
NCBI RefSeq:NC_004102.1
RefSeq ORF Size:1344bp
cDNA Description:Full length Clone DNA of Hepatitis C virus (HCV) (serotype 1a, isolate H77) NS5A with N terminal His tag.
Gene Synonym:HCV-NS5A
Species:HCV
Vector:pCMV3-N-His
Plasmid:
Restriction Site:
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:Identical with the Gene Bank Ref. ID sequence NC_004102.1 (6258..7601), corresponding to amino acid sequence NP_751927.1.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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Catalog: VG40284-NH
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