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Human SerpinB4 Protein (His Tag)

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Human SerpinB4 Protein Product Information
Synonym:LEUPIN, PI11, SCCA-2, SCCA1, SCCA2
Protein Construction:A DNA sequence encoding the human SERPINB4 (P48594) (Met 1-Pro 390) was expressed, with a polyhistidine tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human SerpinB4 Protein QC Testing
Purity:> 88 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human SERPINB4 consists of 409 amino acids and predicts a molecular mass of 47.1 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 20% gly, 1mM EDTA, 1mM DTT
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human SerpinB4 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other SerpinB4 Recombinant Protein Products
Human SerpinB4 / SCCA2 References
  • de Koning PJ, et al. (2011) Intracellular Serine Protease Inhibitor SERPINB4 Inhibits Granzyme M-Induced Cell Death. PLoS One. 6(8): e22645.
  • Chechlinska M, et al. (2010) Squamous cell carcinoma antigen 1 and 2 expression in cultured normal peripheral blood mononuclear cells and in vulvar squamous cell carcinoma. Tumour Biol. 31(6): 559-67.
  • Shiiba M, et al. (2010) Down-regulated expression of SERPIN genes located on chromosome 18q21 in oral squamous cell carcinomas. Oncol Rep. 24(1): 241-9. en, Der p 1. J Biol Chem. 279(7): 5081-7.
  • Murakami A, et al. (2008) Relationship between decreased expression of squamous cell carcinoma antigen 2 and E-cadherin in primary cervical cancer lesions and lymph node metastasis. Oncol Rep. 19(1): 99-104.
  • Silverman GA, et al. (1998) SCCA1 and SCCA2 are proteinase inhibitors that map to the serpin cluster at 18q21.3. Tumour Biol. 19(6): 480-7.
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    Catalog: 12990-H07B-20
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