|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat VRK1 ORF mammalian expression plasmid, C-GFPSpark tag||RG81611-ACG|
|Rat VRK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81611-ACR|
|Rat VRK1 ORF mammalian expression plasmid, N-GFPSpark tag||RG81611-ANG|
|Rat VRK1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG81611-ANR|
|Rat VRK1 ORF mammalian expression plasmid, C-Flag tag||RG81611-CF|
|Rat VRK1 ORF mammalian expression plasmid, C-His tag||RG81611-CH|
|Rat VRK1 ORF mammalian expression plasmid, C-Myc tag||RG81611-CM|
|Rat VRK1 ORF mammalian expression plasmid, C-HA tag||RG81611-CY|
|Rat VRK1 Gene cDNA clone plasmid||RG81611-G|
|Rat VRK1 ORF mammalian expression plasmid, N-Flag tag||RG81611-NF|
|Rat VRK1 ORF mammalian expression plasmid, N-His tag||RG81611-NH|
|Rat VRK1 ORF mammalian expression plasmid, N-Myc tag||RG81611-NM|
|Rat VRK1 ORF mammalian expression plasmid, N-HA tag||RG81611-NY|
|Rat VRK1 natural ORF mammalian expression plasmid||RG81611-UT|
|Learn more about expression Vectors|
VRK1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. Serine/threonine protein kinases are tumor suppressor that controls the activity of AMP-activated protein kinase family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. VRK1 contains 1 protein kinase domain and localizes to the nucleus. VRK1 gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. As a serine/threonine kinase, VRK1 phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1), also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy.