|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat IZUMO4 ORF mammalian expression plasmid, C-GFPSpark tag||RG81606-ACG|
|Rat IZUMO4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81606-ACR|
|Rat IZUMO4 ORF mammalian expression plasmid, C-Flag tag||RG81606-CF|
|Rat IZUMO4 ORF mammalian expression plasmid, C-His tag||RG81606-CH|
|Rat IZUMO4 ORF mammalian expression plasmid, C-Myc tag||RG81606-CM|
|Rat IZUMO4 ORF mammalian expression plasmid, C-HA tag||RG81606-CY|
|Rat IZUMO4 Gene cDNA clone plasmid||RG81606-G|
|Rat IZUMO4 ORF mammalian expression plasmid, N-Flag tag||RG81606-NF|
|Rat IZUMO4 ORF mammalian expression plasmid, N-His tag||RG81606-NH|
|Rat IZUMO4 ORF mammalian expression plasmid, N-Myc tag||RG81606-NM|
|Rat IZUMO4 ORF mammalian expression plasmid, N-HA tag||RG81606-NY|
|Rat IZUMO4 natural ORF mammalian expression plasmid||RG81606-UT|
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Izumo is a sperm membrane protein which plays a key role in the fusion in the mouse. It has an Immunoglobulin (Ig) domain and an N-terminal domain for which neither the functions nor homologous sequences are known. Up to now, there four members has an N-terminal domain with significant homology to the N-terminal domain of Izumo. We call this domain Izumo domain. The four proteins are Izumo 1, 2, 3, and 4. Izumo domain possesses the ability to form dimers, whereas the transmembrane domain or the cytoplasmic domain or both of Izumo 1 are required for the formation of multimers of higher order. Izumo 1-3 are transmembrane proteins expressed specifically in the testis, and Izumo 4 is a soluble protein expressed in the testis and in other tissues. Izumo 1, 3, and 4 formed protein complexes on sperm, Izumo 1 forming several larger complexes and Izumo 3 and 4 forming a single larger complex. Co-immunoprecipitation studies showed the presence of other sperm proteins associated with Izumo 1, suggesting Izumo 1 forms a multiprotein membrane complex.