|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rabbit CD3E ORF mammalian expression plasmid, C-GFPSpark tag||TG65002-ACG|
|Rabbit CD3E ORF mammalian expression plasmid, C-OFPSpark / RFP tag||TG65002-ACR|
|Rabbit CD3E ORF mammalian expression plasmid, C-Flag tag||TG65002-CF|
|Rabbit CD3E ORF mammalian expression plasmid, C-His tag||TG65002-CH|
|Rabbit CD3E ORF mammalian expression plasmid, C-Myc tag||TG65002-CM|
|Rabbit CD3E ORF mammalian expression plasmid, C-HA tag||TG65002-CY|
|Rabbit CD3E Gene cDNA clone plasmid||TG65002-G|
|Rabbit CD3E ORF mammalian expression plasmid, N-Flag tag||TG65002-NF|
|Rabbit CD3E ORF mammalian expression plasmid, N-His tag||TG65002-NH|
|Rabbit CD3E ORF mammalian expression plasmid, N-Myc tag||TG65002-NM|
|Rabbit CD3E ORF mammalian expression plasmid, N-HA tag||TG65002-NY|
|Rabbit CD3E natural ORF mammalian expression plasmid||TG65002-UT|
|Learn more about expression Vectors|
T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.