|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat F10 ORF mammalian expression plasmid, C-GFPSpark tag||RG81157-ACG|
|Rat F10 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81157-ACR|
|Rat F10 ORF mammalian expression plasmid, C-Flag tag||RG81157-CF|
|Rat F10 ORF mammalian expression plasmid, C-His tag||RG81157-CH|
|Rat F10 ORF mammalian expression plasmid, C-Myc tag||RG81157-CM|
|Rat F10 ORF mammalian expression plasmid, C-HA tag||RG81157-CY|
|Rat F10 ORF mammalian expression plasmid, N-Flag tag||RG81157-NF|
|Rat F10 ORF mammalian expression plasmid, N-His tag||RG81157-NH|
|Rat F10 ORF mammalian expression plasmid, N-Myc tag||RG81157-NM|
|Rat F10 ORF mammalian expression plasmid, N-HA tag||RG81157-NY|
|Rat F10 Gene cDNA clone plasmid||RG81157-U|
|Rat F10 natural ORF mammalian expression plasmid||RG81157-UT|
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Coagulation factor X, also known as FX, F10, Eponym Stuart-Prower factor, and thrombokinase, is an enzyme of the coagulation cascade. It is one of the vitamin K-dependent serine proteases, and plays a crucial role in the coagulation cascade and blood clotting, as the first enzyme in the common pathway of thrombus formation. Factor X deficiency is one of the rarest of the inherited coagulation disorders. FX deficiency among the most severe of the rare coagulation defects, typically including hemarthroses, hematomas, and umbilical cord, gastrointestinal, and central nervous system bleeding. Factor X is synthesized in the liver as a mature heterodimer formed from a single-chain precursor, and vitamin K is essential for its synthesis. Factor X is activated into factor Xa (FXa) by both factor IX (with its cofactor, factor VIII in a complex known as intrinsic Xase) and factor VII (with its cofactor, tissue factor in a complex known as extrinsic Xase) through cleaving the activation propeptide. As the first member of the final common pathway or thrombin pathway, FXa converts prothrombin to thrombin in the presence of factor Va, Ca2+, and phospholipid during blood clotting and cleaves prothrombin in two places (an arg-thr and then an arg-ile bond). This process is optimized when factor Xa is complexed with activated cofactor V in the prothrombinase complex. Inborn deficiency of factor X is very uncommon, and may present with epistaxis (nose bleeds), hemarthrosis (bleeding into joints) and gastrointestinal blood loss. Apart from congenital deficiency, low factor X levels may occur occasionally in a number of disease states. Furhermore, factor X deficiency may be seen in amyloidosis, where factor X is adsorbed to the amyloid fibrils in the vasculature.