|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat NPM1 ORF mammalian expression plasmid, C-GFPSpark tag||RG81078-ACG|
|Rat NPM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81078-ACR|
|Rat NPM1 ORF mammalian expression plasmid, N-GFPSpark tag||RG81078-ANG|
|Rat NPM1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG81078-ANR|
|Rat NPM1 ORF mammalian expression plasmid, C-Flag tag||RG81078-CF|
|Rat NPM1 ORF mammalian expression plasmid, C-His tag||RG81078-CH|
|Rat NPM1 ORF mammalian expression plasmid, C-Myc tag||RG81078-CM|
|Rat NPM1 ORF mammalian expression plasmid, C-HA tag||RG81078-CY|
|Rat NPM1 ORF mammalian expression plasmid, N-Flag tag||RG81078-NF|
|Rat NPM1 ORF mammalian expression plasmid, N-His tag||RG81078-NH|
|Rat NPM1 ORF mammalian expression plasmid, N-Myc tag||RG81078-NM|
|Rat NPM1 ORF mammalian expression plasmid, N-HA tag||RG81078-NY|
|Rat NPM1 Gene cDNA clone plasmid||RG81078-U|
|Rat NPM1 natural ORF mammalian expression plasmid||RG81078-UT|
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Nucleophosmin 1 (NPM1), also known as nucleolar phosphoprotein B23 or numatrin, is a member of the nucleoplasmin family. Nucleophosmin (NPM) is a nucleolar phosphoprotein that plays multiple roles in ribosome assembly and transport, cytoplasmic-nuclear trafficking, centrosome duplication and regulation of p53. The NPM1 gene is frequently involved in chromosomal translocation, mutation and deletion. Mutations of the NPM1 gene leading to the expression of a cytoplasmic mutant protein, NPMc+, are the most frequent genetic abnormalities found in acute myeloid leukemias. Acute myeloid leukemias (AML) with mutated NPM1 have distinct characteristics, including a significant association with a normal karyotype, involvement of different hematopoietic lineages, a specific gene-expression profile and clinically, a better response to induction therapy and a favorable prognosis. In addition, NPM1 is a crucial gene to consider in the context of the genetics and biology of cancer. NPM1 is frequently overexpressed, mutated, rearranged and deleted in human cancer. Traditionally regarded as a tumour marker and a putative proto-oncogene, it has now also been attributed with tumour-suppressor functions.