|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat KRT14 ORF mammalian expression plasmid, C-GFPSpark tag||RG80965-ACG|
|Rat KRT14 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80965-ACR|
|Rat KRT14 ORF mammalian expression plasmid, N-GFPSpark tag||RG80965-ANG|
|Rat KRT14 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80965-ANR|
|Rat KRT14 ORF mammalian expression plasmid, C-Flag tag||RG80965-CF|
|Rat KRT14 ORF mammalian expression plasmid, C-His tag||RG80965-CH|
|Rat KRT14 ORF mammalian expression plasmid, C-Myc tag||RG80965-CM|
|Rat KRT14 ORF mammalian expression plasmid, C-HA tag||RG80965-CY|
|Rat KRT14 ORF mammalian expression plasmid, N-Flag tag||RG80965-NF|
|Rat KRT14 ORF mammalian expression plasmid, N-His tag||RG80965-NH|
|Rat KRT14 ORF mammalian expression plasmid, N-Myc tag||RG80965-NM|
|Rat KRT14 ORF mammalian expression plasmid, N-HA tag||RG80965-NY|
|Rat KRT14 Gene cDNA clone plasmid||RG80965-U|
|Rat KRT14 natural ORF mammalian expression plasmid||RG80965-UT|
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Cytokeratin 14, also known as Keratin 14 and K14, is a member of the keratin family. Cytokeratin 14 is a type I keratin. It is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Cytokeratin 14 is mainly expressed in the basal layer. It is also strongly expressed in the outer root sheath of anagen follicles. Cytokeratin 14 and keratin 5 may have a role in maintenance of cell proliferation potential in the basal layer of stratified epithelia, modulating phosphatidylinositol 3-kinase/Akt–mediated cell proliferation and/or Notch1-dependent cell differentiation. Cytokeratin 14 defect prevents it from working effectively with keratin 5 and interfering with the assembly of the keratin intermediate filament network. A disruption in this network makes keratinocytes fragile and prone to rupture. Minor trauma to the skin, such as rubbing or scratching, can cause these cells to break down, resulting in the formation of painful, fluid-filled blisters. Mutations in the K14 gene are also responsible for Naegeli-Franceschetti-Jadassohn syndrome and dermatopathia pigmentosa reticularis.