|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Rat IFITM3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80739-ACG|
|Rat IFITM3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80739-ACR|
|Rat IFITM3 ORF mammalian expression plasmid, C-Flag tag||RG80739-CF|
|Rat IFITM3 ORF mammalian expression plasmid, C-His tag||RG80739-CH|
|Rat IFITM3 ORF mammalian expression plasmid, C-Myc tag||RG80739-CM|
|Rat IFITM3 ORF mammalian expression plasmid, C-HA tag||RG80739-CY|
|Rat IFITM3 ORF mammalian expression plasmid, N-Flag tag||RG80739-NF|
|Rat IFITM3 ORF mammalian expression plasmid, N-His tag||RG80739-NH|
|Rat IFITM3 ORF mammalian expression plasmid, N-Myc tag||RG80739-NM|
|Rat IFITM3 ORF mammalian expression plasmid, N-HA tag||RG80739-NY|
|Rat IFITM3 Gene cDNA clone plasmid||RG80739-U|
|Rat IFITM3 natural ORF mammalian expression plasmid||RG80739-UT|
|Learn more about expression Vectors|
Interferon-induced transmembrane protein 3 (IFITM3) belongs to the CD225 family. To replicate, viruses must gain access to the host cell's resources. Interferon (IFN) regulates the actions of a large complement of interferon effector genes (IEGs) that prevent viral replication. The interferon inducible transmembrane protein family members, IFITM1, 2 and 3, are IEGs required for inhibition of influenza A virus, dengue virus, and West Nile virus replication in vitro. IFITM3 is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. It is both necessary and sufficient for preventing the emergence of viral genomes from the endosomal pathway. Viral pseudoparticles were inhibited from transferring their contents into the host cell cytosol by IFN, and IFITM3 was required and sufficient for this action. IFITM3 overexpression is sufficient for this phenotype. Moreover, IFITM3 partially resides in late endosomal and lysosomal structures, placing it in the path of invading viruses.