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Human CREB3L1 / OASIS (aa 396-519) HEK293 Cell Lysate (WB positive control)

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Human CREB3L1 Transfected / Overexpression Cell Lysate Product Information
Expressed Host:Human Cells
Product Description:Human Cell lysate that Human CREB3L1 / OASIS (aa 396-519) transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
Sequence information:A DNA sequence encoding the human CREB3L1 (Q96BA8-1) (Glu396-Ser519) was expressed with a polyhistidine tag at the C-terminus.
Predicted N Terminal:Glu 396
Molecule Mass:The recombinant human CREB3L1 consists of 135 amino acids and predicts a molecular mass of 15.2 KDa. It migrates as an approximately 21-31 KDa band in SDS-PAGE under reducing conditions.
Human CREB3L1 Transfected / Overexpression Cell Lysate Usage Guide
Preparation Method:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
Stability:Samples are stable for up to twelve months from date of receipt.
Recommend Usage:1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
Storage Buffer:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Storage Instruction:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
Application notes:Western blot (WB): Use at an assay dependent dilution.
Other Applications: Not tested.
Optimal dilutions/concentrations should be determined by the end user.
CREB3L1 / OASIS Background

CREB3L1, also known as OASIS, is a cellular transcription factor synthesized as a membrane-bound precursor. It is a putative endoplasmic reticulum (ER) stress sensor in astrocytes with a mechanism of activation. OASIS mRNA expression was detected in pancreatic β-cell lines and rodent islets, and the expression level was up-regulated by ER stress-inducing compounds. CREB3L1 may have a role in pancreas development. CREB3L1 may also play an important role in limiting virus spread by inhibiting proliferation of virus-infected cells. In vitro, CREB3L1 binds to box-B element, cAMP response element (CRE) and CRE-like sequences, and activates transcription through box-B element but not through CRE. It may play a role in gliosis.

Human CREB3L1 / OASIS References
  • Denard B. et al., 2011, Cell Host Microbe. 10 (1): 65-74.
  • Debelenko LV. et al., 2011, 50 (12): 1054-62.
  • Vellanki. et al., 2010, Endocrinology. 151 (9): 4146.
  • Size / Price
    Catalog: 12712-H08HL-300
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