|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat SFN ORF mammalian expression plasmid, C-GFPSpark tag||RG80554-ACG|
|Rat SFN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80554-ACR|
|Rat SFN ORF mammalian expression plasmid, N-GFPSpark tag||RG80554-ANG|
|Rat SFN ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80554-ANR|
|Rat SFN ORF mammalian expression plasmid, C-Flag tag||RG80554-CF|
|Rat SFN ORF mammalian expression plasmid, C-His tag||RG80554-CH|
|Rat SFN ORF mammalian expression plasmid, C-Myc tag||RG80554-CM|
|Rat SFN ORF mammalian expression plasmid, C-HA tag||RG80554-CY|
|Rat SFN ORF mammalian expression plasmid, N-Flag tag||RG80554-NF|
|Rat SFN ORF mammalian expression plasmid, N-His tag||RG80554-NH|
|Rat SFN ORF mammalian expression plasmid, N-Myc tag||RG80554-NM|
|Rat SFN ORF mammalian expression plasmid, N-HA tag||RG80554-NY|
|Rat SFN Gene cDNA clone plasmid||RG80554-U|
|Rat SFN natural ORF mammalian expression plasmid||RG80554-UT|
|Learn more about expression Vectors|
14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.