|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Rat TPST1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80470-ACG|
|Rat TPST1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80470-ACR|
|Rat TPST1 ORF mammalian expression plasmid, C-Flag tag||RG80470-CF|
|Rat TPST1 ORF mammalian expression plasmid, C-His tag||RG80470-CH|
|Rat TPST1 ORF mammalian expression plasmid, C-Myc tag||RG80470-CM|
|Rat TPST1 ORF mammalian expression plasmid, C-HA tag||RG80470-CY|
|Rat TPST1 ORF mammalian expression plasmid, N-Flag tag||RG80470-NF|
|Rat TPST1 ORF mammalian expression plasmid, N-His tag||RG80470-NH|
|Rat TPST1 ORF mammalian expression plasmid, N-Myc tag||RG80470-NM|
|Rat TPST1 ORF mammalian expression plasmid, N-HA tag||RG80470-NY|
|Rat TPST1 Gene cDNA clone plasmid||RG80470-U|
|Rat TPST1 natural ORF mammalian expression plasmid||RG80470-UT|
|Learn more about expression Vectors|
Protein-tyrosine sulfotransferase 1, also known as Tyrosylprotein sulfotransferase 1 and TPST1, is a single-pass type I I membrane protein which belongs to the protein sulfotransferase family. Tyrosine O-sulfation is a common posttranslational modification of proteins in all multicellular organisms. This reaction is mediated by a Golgi enzyme activity called tyrosylprotein sulfotransferase (TPST) that catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to tyrosine residues within acidic motifs of polypeptides. Tyrosine O-sulfation has been shown to be important in protein-protein interactions in several systems. Tyrosine sulfation is mediated by one of two Golgi isoenzymes, called tyrosylprotein sulfotransferases (TPST-1 and TPST-2). A relatively small number of proteins are known to undergo tyrosine sulfation, including certain adhesion molecules, G-protein-coupled receptors, coagulation factors, serpins, extracellular matrix proteins, and hormones. TPST1 is a human tyrosylprotein sulfotransferase that uses 3'phosphoadenosine-5'phosphosulfate (PAPS) to transfer the sulfate moiety to proteins predominantly designated for secretion. TPST1 bears N-linked glycosyl residues exclusively at position Asn60 and Asn262. TPST1 and TPST2 have distinct biological roles that may reflect differences in their macromolecular substrate specificity.