|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat IL24 ORF mammalian expression plasmid, C-GFPSpark tag||RG80464-ACG|
|Rat IL24 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80464-ACR|
|Rat IL24 ORF mammalian expression plasmid, C-Flag tag||RG80464-CF|
|Rat IL24 ORF mammalian expression plasmid, C-His tag||RG80464-CH|
|Rat IL24 ORF mammalian expression plasmid, C-Myc tag||RG80464-CM|
|Rat IL24 ORF mammalian expression plasmid, C-HA tag||RG80464-CY|
|Rat IL24 Gene cDNA clone plasmid||RG80464-G|
|Rat IL24 ORF mammalian expression plasmid, N-Flag tag||RG80464-NF|
|Rat IL24 ORF mammalian expression plasmid, N-His tag||RG80464-NH|
|Rat IL24 ORF mammalian expression plasmid, N-Myc tag||RG80464-NM|
|Rat IL24 ORF mammalian expression plasmid, N-HA tag||RG80464-NY|
|Rat IL24 natural ORF mammalian expression plasmid||RG80464-UT|
|Learn more about expression Vectors|
Interleukin-24 (IL-24) also known as Melanoma differentiation-associated gene 7 protein (MDA-7) is a member of the IL10 family of cytokines. IL-24/MDA-7/IL24 can induce apoptosis selectively in various cancer cells. Overexpression of IL-24 leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Human IL-24/MDA-7/IL24 is secreted by activated peripheral blood mononuclear cells and is the ligand for two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2. Northern blot analysis revealed IL-24/MDA-7/IL24 expression in human tissues associated with the immune system such as spleen, thymus, peripheral blood leukocytes and normal melanocytes. IL-24/MDA-7/IL24 binding to either its endogenous receptors on human keratinocytes or to ectopically expressed receptors on baby hamster kidney cells leads to activation of the signal transducers and activators of transcription.