|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Rat LAIR1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80415-ACG|
|Rat LAIR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80415-ACR|
|Rat LAIR1 ORF mammalian expression plasmid, C-Flag tag||RG80415-CF|
|Rat LAIR1 ORF mammalian expression plasmid, C-His tag||RG80415-CH|
|Rat LAIR1 ORF mammalian expression plasmid, C-Myc tag||RG80415-CM|
|Rat LAIR1 ORF mammalian expression plasmid, C-HA tag||RG80415-CY|
|Rat LAIR1 Gene cDNA clone plasmid||RG80415-G|
|Rat LAIR1 ORF mammalian expression plasmid, N-Flag tag||RG80415-NF|
|Rat LAIR1 ORF mammalian expression plasmid, N-His tag||RG80415-NH|
|Rat LAIR1 ORF mammalian expression plasmid, N-Myc tag||RG80415-NM|
|Rat LAIR1 ORF mammalian expression plasmid, N-HA tag||RG80415-NY|
|Rat LAIR1 natural ORF mammalian expression plasmid||RG80415-UT|
|Learn more about expression Vectors|
Leukocyte associated Ig-like receptor-1 (LAIR1) is a surface molecule expressed on human mononuclear leukocytes that functions as an inhibitory receptor on human NK cells. In addition to NK cells, LAIR1 is expressed on T cells, B cells, macrophages, and dendritic cells. It is predicted to mediate inhibitory functions based on the presence of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic domain. Cross-linking of LAIR1 on human T cell clones results in inhibition of cytotoxicity only in T cell clones that lack CD28 and are able to spontaneously lyse certain targets in vitro. Moreover, the cytolytic activity of freshly isolated T cells, which is thought to be mainly due to "effector" T cells, can be inhibited by anti-LAIR1 mAb. Thus, LAIR1 functions as an inhibitory receptor not only on NK cells, but also on human T cells. This indicates that LAIR1 provides a mechanism of regulation of effector T cells and may play a role in the inhibition of unwanted bystander responses mediated by Ag-specific T cells.