|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Rat NTRK1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80404-ACG|
|Rat NTRK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80404-ACR|
|Rat NTRK1 ORF mammalian expression plasmid, C-Flag tag||RG80404-CF|
|Rat NTRK1 ORF mammalian expression plasmid, C-His tag||RG80404-CH|
|Rat NTRK1 ORF mammalian expression plasmid, C-Myc tag||RG80404-CM|
|Rat NTRK1 ORF mammalian expression plasmid, C-HA tag||RG80404-CY|
|Rat NTRK1 Gene cDNA clone plasmid||RG80404-G|
|Rat NTRK1 ORF mammalian expression plasmid, N-Flag tag||RG80404-NF|
|Rat NTRK1 ORF mammalian expression plasmid, N-His tag||RG80404-NH|
|Rat NTRK1 ORF mammalian expression plasmid, N-Myc tag||RG80404-NM|
|Rat NTRK1 ORF mammalian expression plasmid, N-HA tag||RG80404-NY|
|Rat NTRK1 natural ORF mammalian expression plasmid||RG80404-UT|
|Learn more about expression Vectors|
TRKA is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors. The presence of NTRK1 leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. It was originally identified as an oncogene as it is commonly mutated in cancers, particularly colon and thyroid carcinomas. TRKA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. NTRK1 has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma.