|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat NTRK1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80404-ACG|
|Rat NTRK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80404-ACR|
|Rat NTRK1 ORF mammalian expression plasmid, C-Flag tag||RG80404-CF|
|Rat NTRK1 ORF mammalian expression plasmid, C-His tag||RG80404-CH|
|Rat NTRK1 ORF mammalian expression plasmid, C-Myc tag||RG80404-CM|
|Rat NTRK1 ORF mammalian expression plasmid, C-HA tag||RG80404-CY|
|Rat NTRK1 Gene cDNA clone plasmid||RG80404-G|
|Rat NTRK1 ORF mammalian expression plasmid, N-Flag tag||RG80404-NF|
|Rat NTRK1 ORF mammalian expression plasmid, N-His tag||RG80404-NH|
|Rat NTRK1 ORF mammalian expression plasmid, N-Myc tag||RG80404-NM|
|Rat NTRK1 ORF mammalian expression plasmid, N-HA tag||RG80404-NY|
|Rat NTRK1 natural ORF mammalian expression plasmid||RG80404-UT|
|Learn more about expression Vectors|
TRKA is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors. The presence of NTRK1 leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. It was originally identified as an oncogene as it is commonly mutated in cancers, particularly colon and thyroid carcinomas. TRKA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. NTRK1 has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma.