|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat TLR4 ORF mammalian expression plasmid, C-GFPSpark tag||RG80389-ACG|
|Rat TLR4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80389-ACR|
|Rat TLR4 ORF mammalian expression plasmid, C-Flag tag||RG80389-CF|
|Rat TLR4 ORF mammalian expression plasmid, C-His tag||RG80389-CH|
|Rat TLR4 ORF mammalian expression plasmid, C-Myc tag||RG80389-CM|
|Rat TLR4 ORF mammalian expression plasmid, C-HA tag||RG80389-CY|
|Rat TLR4 Gene cDNA clone plasmid||RG80389-G|
|Rat TLR4 ORF mammalian expression plasmid, N-Flag tag||RG80389-NF|
|Rat TLR4 ORF mammalian expression plasmid, N-His tag||RG80389-NH|
|Rat TLR4 ORF mammalian expression plasmid, N-Myc tag||RG80389-NM|
|Rat TLR4 ORF mammalian expression plasmid, N-HA tag||RG80389-NY|
|Rat TLR4 natural ORF mammalian expression plasmid||RG80389-UT|
|Learn more about expression Vectors|
TLR4, also known as TLR-4, is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR4 is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. TLR 4 has also been designated as CD284 (cluster of differentiation 284). It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). It acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It is also involved in LPS-independent inflammatory responses triggered by Ni(2+).