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UBCH8 / UBE2L6 Antibody, Rabbit PAb, Antigen Affinity Purified

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Human UBCH8/UBE2L6 Antibody Product Information
Immunogen:Recombinant Human UBCH8 / UBE2L6 protein (Catalog#12641-H07E)
Clone ID:
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human UBCH8 / UBE2L6 (rh UBCH8 / UBE2L6; Catalog#12641-H07E; O14933-1; Met1-Ser153). UBCH8 / UBE2L6 specific IgG was purified by Human UBCH8 / UBE2L6 affinity chromatography.
Human UBCH8/UBE2L6 Antibody Usage Guide
Specificity:Human UBCH8 / UBE2L6
Application:WB, ELISA, IP

WB: 2-5 μg/mL

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human UBCH8 / UBE2L6. The detection limit for Human UBCH8 / UBE2L6 is approximately 0.00245 ng/well.

IP: 1-4 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human UBCH8/UBE2L6 Antibody WB Application Image
UBCH8 / UBE2L6 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
UBCH8 / UBE2L6 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Human UBCH8/UBE2L6 Antibody IP Application Image
UBCH8 / UBE2L6 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
Other UBCH8/UBE2L6 Antibody Products
UBCH8 / UBE2L6 Background

UBCH8, also known as UBE2L6, belongs to the ubiquitin-conjugating enzyme family. The family of ubiquitin-conjugating (E2) enzymes is characterized by the presence of a highly conserved ubiquitin-conjugating (UBC) domain. These domains accommodate the ATP-activated ubiquitin (Ub) or ubiquitin-like (UBL) protein via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the E1 and E3 enzymes and connect activation to covalent modification. By doing so, E2s differentiate effects on downstream substrates, either with a single Ub/UBL molecule or as a chain. UBCH8 is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. It catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. UBCH8 functions in the E6/E6-AP-induced ubiquitination of p53/TP53 and promotes ubiquitination and subsequent proteasomal degradation of FLT3. At protein level, it is present in natural killer cells.

Human UBCH8 / UBE2L6 References
  • Moynihan TP, et al. (1999) The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1. J Biol Chem. 274(43):30963-8.
  • Ardley HC, et al. (2000) Genomic organization of the human ubiquitin-conjugating enzyme gene, UBE2L6 on chromosome 11q12. Cytogenet Cell Genet. 89(1-2):137-40.
  • Zhao C, et al. (2004) The UbcH8 ubiquitin E2 enzyme is also the E2 enzyme for ISG15, an IFN-alpha/beta-induced ubiquitin-like protein. Proc Natl Acad. 101(20):7578-82.
  • Tripathi MK, et al. (2005) Down-regulation of UCRP and UBE2L6 in BRCA2 knocked-down human breast cells. Biochem Biophys Res Commun. 328(1):43-8.
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