|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Rat NT5E ORF mammalian expression plasmid, C-GFPSpark tag||RG80335-ACG|
|Rat NT5E ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80335-ACR|
|Rat NT5E ORF mammalian expression plasmid, C-Flag tag||RG80335-CF|
|Rat NT5E ORF mammalian expression plasmid, C-His tag||RG80335-CH|
|Rat NT5E ORF mammalian expression plasmid, C-Myc tag||RG80335-CM|
|Rat NT5E ORF mammalian expression plasmid, C-HA tag||RG80335-CY|
|Rat NT5E Gene cDNA clone plasmid||RG80335-G|
|Rat NT5E ORF mammalian expression plasmid, N-Flag tag||RG80335-NF|
|Rat NT5E ORF mammalian expression plasmid, N-His tag||RG80335-NH|
|Rat NT5E ORF mammalian expression plasmid, N-Myc tag||RG80335-NM|
|Rat NT5E ORF mammalian expression plasmid, N-HA tag||RG80335-NY|
|Rat NT5E natural ORF mammalian expression plasmid||RG80335-UT|
|Learn more about expression Vectors|
5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.