|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat CLEC4F ORF mammalian expression plasmid, C-GFPSpark tag||RG80215-ACG|
|Rat CLEC4F ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80215-ACR|
|Rat CLEC4F ORF mammalian expression plasmid, C-Flag tag||RG80215-CF|
|Rat CLEC4F ORF mammalian expression plasmid, C-His tag||RG80215-CH|
|Rat CLEC4F ORF mammalian expression plasmid, C-Myc tag||RG80215-CM|
|Rat CLEC4F ORF mammalian expression plasmid, C-HA tag||RG80215-CY|
|Rat CLEC4F Gene cDNA clone plasmid||RG80215-G|
|Rat CLEC4F ORF mammalian expression plasmid, N-Flag tag||RG80215-NF|
|Rat CLEC4F ORF mammalian expression plasmid, N-His tag||RG80215-NH|
|Rat CLEC4F ORF mammalian expression plasmid, N-Myc tag||RG80215-NM|
|Rat CLEC4F ORF mammalian expression plasmid, N-HA tag||RG80215-NY|
|Rat CLEC4F natural ORF mammalian expression plasmid||RG80215-UT|
|Learn more about expression Vectors|
CLEC4F, a member of C-type lectins, was firstly purified from rat liver extract with high binding affinity to fucose, galactose and N-acetylgalactosamine, and un-sialylated glucosphingolipids with GalNAc or Gal terminus. However, the biological functions of CLEC4F have not been elucidated. Histochemical staining showed that mouse CLEC4F（mCLEC4F） is only expressed on F4/80+ cells localized in liver, and is undetectable in bone marrow, spleen, lymph nodes, or other tissues in adult mice. However, mCLEC4F is detected in the liver of embryonic day 11.5 (E11.5), which is 1.5 day earlier than the formation of liver (E10) and is 3.5 day earlier than the formation of bone marrow (E15-16). Moreover, recombinant mCLEC4F.Fc binds to alpha-galactoceramide in a Ca++-dependent manner, and both galactose and ceramide can partially inhibit CLEC4F.Fc binding to alpha-galactoceramide. Interestingly, mCLEC4F-deficient (mCEC4F k/o) mice produced far less cytokines than wild type littermates after intravenous injection ofalpha-galactoceramide. This suggests that mCLEC4F is not only a specific marker for Kupffer cells, but is also critical for the presentation of glycolipid antigen to NKT cells.