|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat CA13 ORF mammalian expression plasmid, C-GFPSpark tag||RG80088-ACG|
|Rat CA13 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80088-ACR|
|Rat CA13 ORF mammalian expression plasmid, N-GFPSpark tag||RG80088-ANG|
|Rat CA13 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80088-ANR|
|Rat CA13 ORF mammalian expression plasmid, C-Flag tag||RG80088-CF|
|Rat CA13 ORF mammalian expression plasmid, C-His tag||RG80088-CH|
|Rat CA13 ORF mammalian expression plasmid, C-Myc tag||RG80088-CM|
|Rat CA13 ORF mammalian expression plasmid, C-HA tag||RG80088-CY|
|Rat CA13 Gene cDNA clone plasmid||RG80088-G|
|Rat CA13 ORF mammalian expression plasmid, N-Flag tag||RG80088-NF|
|Rat CA13 ORF mammalian expression plasmid, N-His tag||RG80088-NH|
|Rat CA13 ORF mammalian expression plasmid, N-Myc tag||RG80088-NM|
|Rat CA13 ORF mammalian expression plasmid, N-HA tag||RG80088-NY|
|Rat CA13 natural ORF mammalian expression plasmid||RG80088-UT|
|Learn more about expression Vectors|
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. The CAXIII is a member of the CA family, which owns a globular molecule with high structural similarity to cytosolic isozymes, CAI, II, and III. Recombinant mouse CAXIII showed catalytic activity similar to those of mitochondrial CAV and cytosolic CAI. In human tissues, CAXIII expression was identified in the thymus, small intestine, spleen, prostate, ovary, colon, and testis. In mouse, positive tissues included the spleen, lung, kidney, heart, brain, skeletal muscle, and testis. In conclusion, the predicted amino acid sequence, structural model, distribution, and activity data suggest that CAXIII represents a novel enzyme, which may play important physiological roles in several organs.