|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat IL25 ORF mammalian expression plasmid, C-GFPSpark tag||RG80194-ACG|
|Rat IL25 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80194-ACR|
|Rat IL25 ORF mammalian expression plasmid, C-Flag tag||RG80194-CF|
|Rat IL25 ORF mammalian expression plasmid, C-His tag||RG80194-CH|
|Rat IL25 ORF mammalian expression plasmid, C-Myc tag||RG80194-CM|
|Rat IL25 ORF mammalian expression plasmid, C-HA tag||RG80194-CY|
|Rat IL25 Gene cDNA clone plasmid||RG80194-G|
|Rat IL25 ORF mammalian expression plasmid, N-Flag tag||RG80194-NF|
|Rat IL25 ORF mammalian expression plasmid, N-His tag||RG80194-NH|
|Rat IL25 ORF mammalian expression plasmid, N-Myc tag||RG80194-NM|
|Rat IL25 ORF mammalian expression plasmid, N-HA tag||RG80194-NY|
|Rat IL25 natural ORF mammalian expression plasmid||RG80194-UT|
|Learn more about expression Vectors|
Interleukin-25 (IL-25) is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. IL-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. IL-25 also regulates the development of autoimmune inflammation mediated by IL-17–producing T cells. IL-25 and IL-17, being members of the same cytokine family, play opposing roles in the pathogenesis of organ-specific autoimmunity. IL-25 promotes cell expansion and Th2 cytokine production when Th2 central memory cells are stimulated with thymic stromal lymphopoietin (TSLP)–activated dendritic cells (DCs), homeostatic cytokines, or T cell receptor for antigen triggering. Elevated expression of IL-25 and IL-25R transcripts was observed in asthmatic lung tissues and atopic dermatitis skin lesions, linking their possible roles with exacerbated allergic disorders. A plausible explanation that IL-25 produced by innate effector eosinophils and basophils may augment the allergic inflammation by enhancing the maintenance and functions of adaptive Th2 memory cells had been provided.