|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat IFNAR1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80180-ACG|
|Rat IFNAR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80180-ACR|
|Rat IFNAR1 ORF mammalian expression plasmid, C-Flag tag||RG80180-CF|
|Rat IFNAR1 ORF mammalian expression plasmid, C-His tag||RG80180-CH|
|Rat IFNAR1 ORF mammalian expression plasmid, C-Myc tag||RG80180-CM|
|Rat IFNAR1 ORF mammalian expression plasmid, C-HA tag||RG80180-CY|
|Rat IFNAR1 Gene cDNA clone plasmid||RG80180-G|
|Rat IFNAR1 ORF mammalian expression plasmid, N-Flag tag||RG80180-NF|
|Rat IFNAR1 ORF mammalian expression plasmid, N-His tag||RG80180-NH|
|Rat IFNAR1 ORF mammalian expression plasmid, N-Myc tag||RG80180-NM|
|Rat IFNAR1 ORF mammalian expression plasmid, N-HA tag||RG80180-NY|
|Rat IFNAR1 natural ORF mammalian expression plasmid||RG80180-UT|
|Learn more about expression Vectors|
Interferon-alpha/beta receptor alpha chain (IFNAR1) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. Tyk2 slows down IFNAR1 degradation and that this is due, at least in part, to inhibition of IFNAR1 endocytosis. Mutant versions of IFNAR1, in which Tyr466 is changed to phenylalanine, can act in a dominant negative manner to inhibit phosphorylation of STAT2. These observations are consistent with a model in which IFNAR1 mediates the interaction between JAK kinases and the STAT transcription factors.