|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat TNFRSF9 ORF mammalian expression plasmid, C-GFPSpark tag||RG80176-ACG|
|Rat TNFRSF9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80176-ACR|
|Rat TNFRSF9 ORF mammalian expression plasmid, C-Flag tag||RG80176-CF|
|Rat TNFRSF9 ORF mammalian expression plasmid, C-His tag||RG80176-CH|
|Rat TNFRSF9 ORF mammalian expression plasmid, C-Myc tag||RG80176-CM|
|Rat TNFRSF9 ORF mammalian expression plasmid, C-HA tag||RG80176-CY|
|Rat TNFRSF9 Gene cDNA clone plasmid||RG80176-G|
|Rat TNFRSF9 ORF mammalian expression plasmid, N-Flag tag||RG80176-NF|
|Rat TNFRSF9 ORF mammalian expression plasmid, N-His tag||RG80176-NH|
|Rat TNFRSF9 ORF mammalian expression plasmid, N-Myc tag||RG80176-NM|
|Rat TNFRSF9 ORF mammalian expression plasmid, N-HA tag||RG80176-NY|
|Rat TNFRSF9 natural ORF mammalian expression plasmid||RG80176-UT|
|Learn more about expression Vectors|
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with the tumor necrosis factor receptor–associated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials.