After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

Human GAD67 / GAD1 Protein (His Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Human GAD1 Protein Product Information
Synonym:FLJ45882, GAD, SCP
Protein Construction:A DNA sequence encoding the human GAD1 (Q99259-1) (Met 1-Leu 594) was fused with a polyhistidine tag at the C-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human GAD1 Protein QC Testing
Purity:> 92 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met 1
Molecule Mass:The recombinant human GAD1 consists of 605 amino acids and predicts a molecular mass of 68.3 kDa. It migrates as an approximately 64 kDa band in SDS-PAGE in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.5
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human GAD1 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other GAD1 Recombinant Protein Products
GAD67/GAD1 Background

Glutamate decarboxylase 1, also known as 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform and GAD1, is a member of the group II decarboxylase family. GAD1 is expressed in benign and malignant prostatic tissue and may serve as a highly prostate-specific tissue biomarker. GAD1 isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Tissue-specific markers are useful for identification of tumour type in advanced cancers of unknown origin. In plants, as in most eukaryotes, glutamate decarboxylase catalyses the synthesis of GABA. Root-specific calcium/calmodulin-regulated GAD1 plays a major role in GABA synthesis in plants under normal growth conditions and in response to stress. Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1)which is a non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis.

Human GAD67/GAD1 References
  • Bouché,N. et al., 2004, Plant Mol Biol. 55 (3):315-25.
  • Huang,H.S. et al., 2007, PLoS One. 2 (8):e809.
  • Fenalti G., et al., 2007, Nat. Struct. Mol. Biol. 14:280-286.
  • Jaraj,S.J. et al., 2011,Scand J Urol Nephrol. 45 (1):39-45.
  • Product nameProduct name
    Size / Price
    Catalog: 12570-H08B-20
    List Price:   (Save )
    Price:      [How to order]
    AvailabilityIn StockShipping instructions